HLA B7 antibody (FITC)

Catalog No. ABIN118933

This Mouse Monoclonal antibody specifically detects HLA B7 in FACS. It exhibits reactivity toward Human.

Quick Overview for HLA B7 antibody (FITC) (ABIN118933)

Target: HLA B7 (HLA Class I B7 Alpha (HLA B7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA B7 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: BB7-1

Product Details anti-HLA B7 Antibody (FITC)

Specificity: This antibody recognises the Human HLA-B7 antigen and does not cross-react with HLA-B27 or other related HLA antigens. It can be used to distinguish true HLA-B27 positives from false HLA-B27 positives (i.e. HLA-B7 positive) in the investigation of such diseases as ankylosing spondylitis and anterior uveitis.

Cross-Reactivity (Details): Species reactivity (expected):Cynomolgus monkey.
Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G

Immunogen: Papain solubilized HLA-B7 antigen.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: use 10 μL of neat - 1/10 diluted antibody to label 1x10^6 cells in 100 μL whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HLA B7

Target: HLA B7 (HLA Class I B7 Alpha (HLA B7))

Background: HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa. Hundreds of HLA B alleles have been described. The HLA B gene is part of the human HLA complex on chromosome 6 and there are a large number of variant alleles of this gene.Synonyms: B-7 alpha chain, HLA class I histocompatibility antigen, HLAB, MHC class I antigen B7

Gene ID: 3106

NCBI Accession: NP_005505

UniProt: P01889

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response