IL12B antibody

Catalog No. ABIN118938

The Mouse Monoclonal anti-IL12B antibody (Clone 1-1A4) (ABIN118938) specifically detects IL12B in IHC (fro) and EIA. The antibody is reactive with Human samples.

Quick Overview for IL12B antibody (ABIN118938)

Target: IL12B (Interleukin 12 beta (IL12B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL12B antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 1-1A4

Product Details anti-IL12B Antibody

Specificity: This antibody recognises the p40 subunit of interleukin-12, and binds to both the free subunit and the p35/p40 heterodimer. The antibody reacts with both stimulated and unstimulated monocytes in immunohistology.

Purification: Purified

Immunogen: Recombinant IL-12. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2-O myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections. ELISA: 1/1000 as detecting antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IL12B

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: Interleukin-12 beta / IL12B

Background: Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10, homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.Synonyms: CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, Interleukin-12B, NK cell stimulatory factor chain 2, NKSF2

Gene ID: 3593

NCBI Accession: NP_002178

UniProt: P29460

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation