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Cytokeratin 18 antibody

KRT18 Reactivity: Human WB, IF, IHC (p), IP, EIA Host: Mouse Monoclonal DC-10 unconjugated
Catalog No. ABIN118968
  • Target See all Cytokeratin 18 (KRT18) Antibodies
    Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
    Reactivity
    • 252
    • 151
    • 112
    • 44
    • 35
    • 28
    • 25
    • 21
    • 19
    • 18
    • 17
    • 14
    • 14
    • 14
    • 9
    • 6
    • 6
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    Human
    Host
    • 165
    • 122
    • 3
    • 1
    • 1
    Mouse
    Clonality
    • 151
    • 141
    Monoclonal
    Conjugate
    • 144
    • 26
    • 22
    • 11
    • 9
    • 9
    • 9
    • 9
    • 9
    • 9
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This Cytokeratin 18 antibody is un-conjugated
    Application
    • 217
    • 100
    • 84
    • 78
    • 74
    • 60
    • 54
    • 40
    • 39
    • 27
    • 26
    • 19
    • 8
    • 8
    • 7
    • 6
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
    Specificity
    The antibody reacts with Human Cytokeratin 18 (45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
    Purification
    Affinity Chromatography on Protein A
    Immunogen
    Human breast carcinoma cell line PMC-42
    Clone
    DC-10
    Isotype
    IgG1
    Top Product
    Discover our top product KRT18 Primary Antibody
  • Application Notes
    Immunoprecipitation. Western Blotting (non-reducing conditions): 1-2 mg/mL, overnight in 4 °C. Positive control: HeLa human cervix carcinoma cell line MCF-7 human breast adenocarcinoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. SDS-PAGE (10 % separating gel). Immunohistochemistry (paraffin sections): 10 - 1:100. Positive tissue: epithelial tissue (skin). Immunocytochemistry. Positive control: MCF-7 human breast adenocarcinoma cell line HeLa human cervix carcinoma cell line. ELISA. Positive control: MCF-7 human breast adenocarcinoma cell line. Application note: The antibody DC-10 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the clone C-04.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS, pH ~7.4, 15 mM Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the original vial at 2-8 °C. Stock solution and aliquots thereof should be stored at -20 °C.
  • Blumenthal, Giebel, Ummanni, Schlüter, Endlich, Endlich: "Morphology and migration of podocytes are affected by CD151 levels." in: American journal of physiology. Renal physiology, Vol. 302, Issue 10, pp. F1265-77, (2012) (PubMed).

    Hachiya, Sriwiriyanont, Fujimura, Ohuchi, Kitahara, Takema, Kitzmiller, Visscher, Tsuboi, Boissy: "Mechanistic effects of long-term ultraviolet B irradiation induce epidermal and dermal changes in human skin xenografts." in: The American journal of pathology, Vol. 174, Issue 2, pp. 401-13, (2009) (PubMed).

    Kirby, Beattie, Maroof, van Rooijen, Kaye: "SIGNR1-negative red pulp macrophages protect against acute streptococcal sepsis after Leishmania donovani-induced loss of marginal zone macrophages." in: The American journal of pathology, Vol. 175, Issue 3, pp. 1107-15, (2009) (PubMed).

    Lau, Mollnau, Eiserich, Freeman, Daiber, Gehling, Brümmer, Rudolph, Münzel, Heitzer, Meinertz, Baldus: "Myeloperoxidase mediates neutrophil activation by association with CD11b/CD18 integrins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 2, pp. 431-6, (2005) (PubMed).

    Vojt?sek, Stasková, Nenutil, Lauerová, Kovarík, Rejthar, Bártková, Bártek: "Monoclonal antibodies recognizing different epitopes of cytokeratin No.18." in: Folia biologica, Vol. 35, Issue 6, pp. 373-82, (1990) (PubMed).

    Kovarík, Rejthar, Lauerová, Vojt?sek, Bártková: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, (1989) (PubMed).

  • Target
    Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
    Alternative Name
    Cytokeratin 18 (KRT18 Products)
    Synonyms
    CK18 antibody, K18 antibody, Krt1-18 antibody, CYK18 antibody, DreK18 antibody, cb83 antibody, dapk1 antibody, sb:cb83 antibody, wu:fa13f02 antibody, wu:fb36b04 antibody, krt18 antibody, MGC64569 antibody, MGC75922 antibody, KRT18 antibody, CK-18-A antibody, K18-A antibody, NC-11 antibody, cyk18 antibody, k18 antibody, krt18-a antibody, krt18-b antibody, krt18a antibody, xkendob antibody, keratin 18 antibody, keratin 18, type I L homeolog antibody, keratin 18, type I antibody, keratin, type I cytoskeletal 18 antibody, K18, simple type I keratin antibody, Keratin, type I cytoskeletal 18 antibody, keratin 18, type I S homeolog antibody, Krt18 antibody, KRT18 antibody, krt18 antibody, krt18.L antibody, LOC700569 antibody, LOC100136778 antibody, k1c18 antibody, LOC100008885 antibody, krt18.S antibody
    Background
    Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18
    Gene ID
    3875
    NCBI Accession
    NP_000215
    UniProt
    P05783
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis
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