Huntingtin antibody (AA 2703-2911)

Catalog No. ABIN119307

This anti-Huntingtin antibody is a Mouse Monoclonal antibody detecting Huntingtin in WB, IP and IHC (fro). Suitable for Human, Mouse and Rabbit.

Quick Overview for Huntingtin antibody (AA 2703-2911) (ABIN119307)

Target: Huntingtin (HTT)

Reactivity: Human, Mouse, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This Huntingtin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: HDC8A4

Product Details anti-Huntingtin Antibody

Binding Specificity: AA 2703-2911

Specificity: This antibody reacts with an epitope corresponding to the HDC region (2703 - 2911 amino acids) of the huntingtin protein. Clone HDC8A4 detects a 350KD band on western blots but also detects smaller degradation products of huntingtin. Clone HDC8A4 recognises both denatured and native huntingtin in human brain. The combined use of clone HDC8A4 (SM1662), HDB4E10 (SM1661) and HDA3E10 (SM1660) demonstrate that huntingtin is enriched in neuronal cells in the brain.

Purification: Affinity chromatography on Protein G

Immunogen: Recombinant protein corresponding to amino acids 2703 - 2911 of huntingtin

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Huntingtin

Target: Huntingtin (HTT)

Alternative Name: Huntingtin

Background: Huntington's disease (HD) is a neurodegenerative disorder caused by an expanding polyglutamine repeat in the huntingtin gene. HD is a mid-life onset autosomal dominant neurodegeneative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years. The HD gene product is widely expressed in human tissues, with the highest level of expression in the brain. The huntingtin gene product is expressed at similar levels in patients and controls, which suggests that the expansion of the polyglutamine repeat induces a toxic gain of function perhaps through interactions with other cellular proteins. Using yeast two-hybrid system, HAP1 (huntingtin associated protein 1) has been identified, that associates with huntingtin protein. The In vitro data suggest that the association between HAP1 and huntingtin is enhanced by increasing length of glutamine repeat.Synonyms: HD, HTT, Huntington Disease Protein, IT15

Gene ID: 3064

NCBI Accession: NP_002102

UniProt: P42858

Pathways: PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport