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SIRPA antibody (FITC)
SIRPA
Reactivity: Rat
FACS
Host: Mouse
Monoclonal
ED9
FITC
Product Details anti-SIRPA Antibody (FITC)
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Target
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SIRPA
(Signal-Regulatory Protein alpha (SIRPA))
Reactivity
All reactivities for SIRPA antibodies
Rat
Host
All hosts for SIRPA antibodies
Mouse
Clonality
All clonalities for SIRPA antibodies
Monoclonal
Conjugate
All conjugates for SIRPA antibodies
This SIRPA antibody is conjugated to FITC
Application
All applications for SIRPA antibodies
Flow Cytometry (FACS)
Specificity
This antibody recognises CD172a, also known as Signal regulatory protein (SIRP). ED9 binds to a different epitope on SIRP than OX41.
Purification
Affinity chromatography on Protein A
Immunogen
Spleen cell homogenate. Spleen cells from immunised mice were fused with cells of the Sp2/0 Ag-14 myeloma cell line.
Clone
ED9
Isotype
IgG1
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Discover our top product SIRPA Primary Antibody
Alternatives
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Application Details
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Application Notes
Flow cytometry: neat - 1/10, use 10 μL of the suggested working dilution to label 10^6 cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Handling
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Concentration
0.1 mg/mL
Buffer
Contains 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target Details for SIRPA
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Target
SIRPA
(Signal-Regulatory Protein alpha (SIRPA))
Alternative Name
CD172a / SIRPA (SIRPA Products )
Synonyms
BIT antibody, CD172A antibody, MFR antibody, MYD-1 antibody, P84 antibody, PTPNS1 antibody, SHPS1 antibody, SIRP antibody, SIRPA antibody, SIRP-A1 antibody, SIRP-B1 antibody, AI835480 antibody, Bit antibody, CD172a antibody, Ptpns1 antibody, SHP-1 antibody, SHPS-1 antibody, swc3 antibody, signal regulatory protein alpha antibody, myeloperoxidase antibody, signal-regulatory protein alpha antibody, tyrosine-protein phosphatase non-receptor type substrate 1 antibody, SIRPA antibody, MPO antibody, LOC458039 antibody, LOC100400378 antibody, LOC100590224 antibody, Sirpa antibody
Background
CD172a is expressed selectively by myeloid cells and by neurons.Synonyms: BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, MFR, MYD1, Macrophage fusion receptor, MyD-1 antigen, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, Signal-regulatory protein alpha-1, Sirp-alpha-2, Sirp-alpha-3, Tyrosine-protein phosphatase non-receptor type substrate 1, p84
Gene ID
25528
NCBI Accession
NP_037148
UniProt
P97710
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