Aquaporin 1 antibody (AA 249-269)

Catalog No. ABIN119568

The Mouse Monoclonal anti-Aquaporin 1 antibody (Clone 1-A5F6) (ABIN119568) specifically detects Aquaporin 1 in WB, IP, IHC (p), IHC (fro) and EIA. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Aquaporin 1 antibody (AA 249-269) (ABIN119568)

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Aquaporin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 1-A5F6

Product Details anti-Aquaporin 1 Antibody

Binding Specificity: AA 249-269

Specificity: This antibody recognises an epitope within the cytoplasmic domain of the water-specific channel Aquaporin 1.

Purification: Affinity Chromatography on Protein G.

Immunogen: Synthetic peptide corresponding to amino acids 249-269 of Rat Aquaporin 1. Spleen cells from immunised Balb/c mice were fused with the cells of the Sp2/0 Ag14 mouse myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation: 1/1000 - 1/10000. Western Blot: 1/1000 - 1/5000. ELISA: 1/1000 - 1/20000. Immunohistochemistry on frozen and paraffin embedded sections: 1/500 - 1/1000. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended positive control tissue: Kidney.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Aquaporin 1

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Alternative Name: Aquaporin-1

Background: Aquaporin 1 is a 28kD integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. The glycosylated forms of AQP1 range between 40-60kD in mass. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.Synonyms: AQP-1, AQP1, Aquaporin-CHIP, CHIP28, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule

Gene ID: 358

NCBI Accession: NP_932766

UniProt: P29972

Pathways: Hormone Transport