CD4 antibody

Catalog No. ABIN120326

The Mouse Monoclonal anti-CD4 antibody is suitable to detect CD4 in samples from Duck. It has been validated for FACS and IP.

Quick Overview for CD4 antibody (ABIN120326)

Target: CD4

Reactivity: Duck

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: Du CD4-2

Product Details anti-CD4 Antibody

Purification: Affinity Chromatography on Protein G.

Immunogen: 293T cells expressing Pekin duck CD4. Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: CD4 is shown to be expressed by thymocytes, splenocytes and peripheral lymphoid cells. Since the majority of avian immune studies have been carried out on chickens, relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs, the spleen, thymus and bursa of Fabricius. At the cellular level, studies have shown that like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin (PHA), and all cells reacting with clone Du CD4-2 have been identified as CD3+ T cells. (1)Synonyms: T-Cell Surface Antigen T4/Leu-3, T-cell Surface Glycoprotein CD4, T4/Leu-3

Gene ID: 8839

UniProt: Q50EU7

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events