CD8B antibody

Catalog No. ABIN120327

The Mouse Monoclonal anti-CD8B antibody (Clone Du CD8-1) (ABIN120327) specifically detects CD8B in FACS and IP. The antibody is reactive with Duck samples.

Quick Overview for CD8B antibody (ABIN120327)

Target: CD8B (CD8b Molecule (CD8B))

Reactivity: Duck

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8B antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: Du CD8-1

Product Details anti-CD8B Antibody

Specificity: This antibody recognises CD8 alpha. Double-staining with clone Du CD8-1 and duck IgY light chain clone 14A3 (product code SM2274P), revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+/14A3+ cells in duck bursa. (1)

Purification: Affinity Chromatography on Protein G.

Immunogen: 293T cells expressing Pekin Duck CD8 alpha. Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD8B

Target: CD8B (CD8b Molecule (CD8B))

Alternative Name: CD8 beta

Background: CD8 alpha shown to be expressed by thymocytes, splenocytes, peripheral lymphoid cells and the vast majority of bursal cells. Since the majority of avian immune studies have been carried out on chickens, relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs: the spleen, thymus and bursa of Fabricius. At the cellular level, studies have shown that like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin (PHA).Synonyms: CD8B, CD8B1, T-cell surface glycoprotein CD8 beta chain

Gene ID: 396175

NCBI Accession: NP_990578

UniProt: Q90769

Pathways: TCR Signaling