CD4 antibody (Domain 1) (APC)

Catalog No. ABIN120373

This Mouse Monoclonal antibody specifically detects CD4 in FACS. It exhibits reactivity toward Rat.

Quick Overview for CD4 antibody (Domain 1) (APC) (ABIN120373)

Target: CD4

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: W3-25

Product Details anti-CD4 Antibody (APC)

Binding Specificity: Domain 1

Immunogen: Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1/5 - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: RT/4 °C/-20 °C

Storage Comment: Lyophilized: Store at 2-8 °C (preferably in a dessicator). Reconstituted: Aliquot and store at -20 °C.

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: CD4 is a 55kD molecule expressed by helper T cells and weakly by monocytes.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Gene ID: 10116

UniProt: P05540

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events