This monoclonal antibody recognizes the smaller (alpha subunit) 55 kDa chain of the IL-2 receptor found on activated rat T cells, thymic dendritic cells but not resting lymphocytes. This antibody binds to the rat interleukin-2 receptor designated CD25 and has proven to be an important marker for activated T cells.
Purification
Protein G Chromatography
Immunogen
T blasts from a mixed lymphocyte reaction between purified CD4 positive T cells and irradiated spleens. Donor: BALB/c spleen Fusion Partner: NSO/1
IL2RA
Reactivity: Human
WB, IHC, ELISA, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Flow cytometry. (Reported to be useful in Immunohistochemistry on frozen and paraffin sections.) Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1,0 mg/mL
Buffer
PBS and 0.02 % NaN3
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55