CD25 antibody (PE)

Catalog No. ABIN120430

This Mouse Monoclonal antibody specifically detects CD25 in FACS. It exhibits reactivity toward Rat.

Quick Overview for CD25 antibody (PE) (ABIN120430)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD25 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: OX-39

Product Details anti-CD25 Antibody (PE)

Specificity: Recognizes the alpha chain of rat CD25, otherwise know as IL-2 receptor alpha, a 55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts (1).

Purification: Protein G purified immunoglobulin fraction

Immunogen: Con A stimulated Rat T cells.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: use 10 μL of neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Reconstitute with 1 mL distilled water.

Buffer: PBS pH 7.4 with 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.

Expiry Date: 6 months

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25 / IL2RA

Background: The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55

Gene ID: 25704

NCBI Accession: NP_037295

UniProt: P26897

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation