KRT8 antibody

Catalog No. ABIN120663

Cited in 1 publication. The Mouse Monoclonal anti-KRT8 antibody (Clone C-43) (ABIN120663) specifically detects KRT8 in WB, IF, IHC (p) and IP. The antibody is reactive with Human, Cow, Rabbit, Pig and Sheep samples.

Quick Overview for KRT8 antibody (ABIN120663)

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human, Cow, Rabbit, Pig, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: C-43

Product Details anti-KRT8 Antibody

Specificity: The antibody C-43 reacts with Cytokeratin 8 (52.5 kDa).

Purification: Affinity Chromatography on Protein A

Immunogen: Cytoskeleton preparation from HeLa human cervix carcinoma cell line

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation. Western Blot. Immunocytochemistry. Immunohistochemistry (Paraffin): 20 m g/mL. Positive tissue: prostate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

References for anti-KRT8 antibody (ABIN120663)

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Cytokeratin 8

Background: Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.Synonyms: CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, type II cytoskeletal 8

Molecular Weight: 52.5 kDa

Gene ID: 3856

UniProt: P05787