ACRV1 antibody

Catalog No. ABIN120688

This Mouse Monoclonal antibody specifically detects ACRV1 in WB and IF. It exhibits reactivity toward Dog.

Quick Overview for ACRV1 antibody (ABIN120688)

Target: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Reactivity: Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACRV1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: Ds-2

Product Details anti-ACRV1 Antibody

Specificity: The antibody reacts with intra-acrosomal proteins (92 and 42 kDa) of sperms.

Characteristics: Synonyms: Acrosomal protein SP-10, SP10, SPACA2, Acrosomal vesicle protein 1, Intra-acrosomalSperm Protein

Purification: Protein-A affinity chromatography

Purity: > 95 % pure (by SDS-PAGE)

Immunogen: Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10%glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0. 2% acetic acid,lyophylized and subsequently used for immunization.

Isotype: IgG

Application Details

Application Notes: Immunocytochemistry, membrane permeabilization (acetone) essential. Western Blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ACRV1

Target: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Alternative Name: ACRV1

Background: Canine intra-acrosomal sperm protein (92 and 42 kDa) is a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.Synonyms: Acrosomal protein SP-10, Acrosomal vesicle protein 1, Intra-acrosomal Sperm Protein, SP10, SPACA2

Gene ID: 56

UniProt: P26436