Acrosin antibody

Catalog No. ABIN120729

The Mouse Monoclonal anti-Acrosin antibody (Clone ACR2) (ABIN120729) specifically detects Acrosin in WB and IF. The antibody is reactive with Pig samples.

Quick Overview for Acrosin antibody (ABIN120729)

Target: Acrosin (ACR)

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Acrosin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: ACR2

Product Details anti-Acrosin Antibody

Specificity: The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa) in the acrosome of undamaged spermatozoa.

Purification: Protein A affinity chromatography

Purity: > 95 % (by SDS-PAGE)

Immunogen: Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization

Isotype: IgG1

Application Details

Application Notes: Immunocytochemistry, membrane permeabilization (acetone) essential. Western Blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for Acrosin

Target: Acrosin (ACR)

Alternative Name: Acrosin / ACR

Background: Acrosin is stored in the acrosome of undamaged spermatozoa.Synonyms: 53 kDa fucose-binding protein

Gene ID: 397098

UniProt: P08001

Pathways: cAMP Metabolic Process