Cyclin D1 antibody

Catalog No. ABIN121518

This anti-Cyclin D1 antibody is a Rabbit Polyclonal antibody detecting Cyclin D1 in WB and EIA. Suitable for Human, Mouse and Rat.

Quick Overview for Cyclin D1 antibody (ABIN121518)

Target: Cyclin D1 (CCND1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Cyclin D1 Antibody

Specificity: This is an antiserum produced by delipidation and defibrination. Reactivity occurs against human Cyclin D1 protein. Expect cross reactivity with Cyclin D1 from many other organisms as the protein, or domains within the protein, are highly homologous in human, chimpanzee, orangutan, rat, mouse, dog, chicken, frog, and zebrafish.

Purification: Delipidation and defibrination.

Immunogen: Full length GST fusion protein corresponding to the human cyclin D1 gene

Application Details

Application Notes: Suitable for se in ELISA (1/500-1/2000) and Western blot(1/500-1/1000). Expect a band approximately 34 kDa in size corresponding to Cyclin D1 by western blotting in the appropriate cell lysate or extract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Human Cyclin D1, 295 aa MW 33.7 kDa

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85 mg/mL (by Refractometry)

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or at -20 °C for longer. This product should be stored undiluted. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: Cyclin D1

Background: Cyclin D1 (also known as G1/S-specific cyclin D1, PRAD1 oncogene, and PRAD1: parathyroid adenomatosis 1) is encoded by a gene that belongs to the highly conserved cyclin family. Cyclins are characterized by a dramatic periodicity in protein abundance throughout the cell cycle and function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns, which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.Synonyms: BCL-1 oncogene, BCL1, CCND1, Cyclin-D1, G1/S-specific cyclin-D1, PRAD-1 oncogene, PRAD1

UniProt: P24385

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling