Calcitonin Receptor antibody

Catalog No. ABIN121621

This Rabbit Polyclonal antibody specifically detects Calcitonin Receptor in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Rat and Mouseand has been mentioned in 1 publication.

Quick Overview for Calcitonin Receptor antibody (ABIN121621)

Target: Calcitonin Receptor (CALCR)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calcitonin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Calcitonin Receptor Antibody

Specificity: SP1338P reacts with the Calcitonin Receptor. It recognises an epitope within the cytoplasmic domain that is common to both C1a and C1b Rat isoforms.

Cross-Reactivity (Details): Species reactivity (tested):Rat. Cross reacts with Mouse and Human.

Purification: Affinity Chromatography on Protein A.

Immunogen: Synthetic peptide derived from sequence of rat calcitonin receptor

Application Details

Application Notes: Immunohistochemistry on paraffin embedded tissues (1:10 - 1:100): The use of 4 % paraformaldehyde fixation rather than formalin fixation is recommended. Does not require protein digestion pre-treatment nor antigen retrieval using heat prior to staining of paraffin embedded sections. Suitable positive control: Kidney, cultered osteoclasts. A sensitive detection system is also recommended, e.g. a catalysed signal amplification system). ELISA (1:10,000). Western blot using reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

References for anti-Calcitonin Receptor antibody (ABIN121621)

Segovia-Silvestre, Bonnefond, Sondergaard, Christensen, Karsdal, Bay-Jensen: "Identification of the calcitonin receptor in osteoarthritic chondrocytes." in: BMC research notes, Vol. 4, pp. 407, (2011) (PubMed).

Target Details for Calcitonin Receptor

Target: Calcitonin Receptor (CALCR)

Alternative Name: Calcitonin Receptor

Background: Calcitonin Receptor is a seven transmembrane domain cell surface protein which is coupled to G protein messenger systems. Calcitonin receptor is expressed in kidney, central and peripheral nervous systems and in osteoclasts. It is also expressed in a number of tissues during the development of the embyro and may be important in foetal morphogenesis.Synonyms: CALCR, CT-R, CTR

Gene ID: 116506

UniProt: P32214

Pathways: cAMP Metabolic Process