Serotonin Receptor 2B antibody (Extracellular Domain)

Catalog No. ABIN121985

The Rabbit Polyclonal anti-Serotonin Receptor 2B antibody has been validated for IHC (p). It is suitable to detect Serotonin Receptor 2B in samples from Human, Mouse and Rat.

Quick Overview for Serotonin Receptor 2B antibody (Extracellular Domain) (ABIN121985)

Target: Serotonin Receptor 2B (HTR2B)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 2B antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Serotonin Receptor 2B Antibody

Binding Specificity: Extracellular Domain

Specificity: This antibody reacts with 5-HT2B Receptor (family: GPCR, subfamily: Serotonin)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Immunohistochemistry with Formalin Fixed Paraffin Embedded Tissues: 4-6 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C forlonger. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Serotonin Receptor 2B

Target: Serotonin Receptor 2B (HTR2B)

Background: 5-HT2B, a Serotonin Receptor, activates phospholipase C upon binding serotonin, which results in a rise in intracellular calcium. It is known that 5-HT2B regulates cardiovascular function during development and in adulthood, mice lacking functional 5-HT2B receptors died of heart defects during gestation or neonatally, and adult mutant mice displayed cardiopathy, including myocyte disarray and ventricular dilation. Abnormal vascular proliferation causes an increase in pulmonary blood pressure, which is associated with an increase in 5-HT2B receptor, resulting in the development of pulmonary hypertension. It has also been reported that the 5-HT2B is involved in cell-cycle regulation via interaction with tyrosine kinase pathways. 5-HT2B receptors may have a role in the initiation of migraine headaches, and selective antagonists may prove therapeutic in migraine treatment. 5-HT2B receptor is expressed in many tissues including human liver, kidney, lung, heart, pancreas, spleen, brain, spinal cord, gastrointestinal tract, placenta, and coronary and pulmonary arteries. It has also been reported in embryonic mouse heart and spinal cord. ESTs have been isolated from heart/melanocyte/uterus, kidney, prostate, skin, and thyroid libraries.Synonyms: 5-HT-2B, 5-HT2B, 5-hydroxytryptamine receptor 2B

Gene ID: 3357

UniProt: P41595

Pathways: JAK-STAT Signaling, Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Carbohydrate Metabolic Process