CALCRL antibody (Cytoplasmic Domain)

Catalog No. ABIN122018

The Rabbit Polyclonal anti-CALCRL antibody has been validated for IHC (p). It is suitable to detect CALCRL in samples from Human, Mouse and Rat.

Quick Overview for CALCRL antibody (Cytoplasmic Domain) (ABIN122018)

Target: CALCRL (Calcitonin Receptor-Like (CALCRL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CALCRL antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CALCRL Antibody

Binding Specificity: Cytoplasmic Domain

Specificity: Reacts with Calcitonin Receptor-Like Receptor (Family: GPCR, subfamily: Adrenomedullin). BLAST analysis of the peptide immunogen showed no homology with other Human proteins, except CALCR (56 %).

Cross-Reactivity (Details): Species reactivity (expected):Mouse (93 % identity and similarity) and Rat (100 % identity and similarity).
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Immunohistochemistry with formalin fixed paraffin embedded tissues: 34 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C forlonger. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CALCRL

Target: CALCRL (Calcitonin Receptor-Like (CALCRL))

Alternative Name: CGRP Type 1 Receptor / CRLR

Background: Calcitonin receptor-like receptor (CRLR) is an Adrenomedullin Receptor that requires two additional proteins to function: a chaperone protein RAMP (receptor activity modifying protein) and the component protein RCP. The function of CRLR depends on its interaction with RAMP. When coexpressed with RAMP1, CRLR acts as a receptor for calcitonin gene-related peptide (CGRP). In contrast, when CRLR is coexpressed with either RAMP2 or RAMP3, it acts as a receptor for adrenomedullin. Activation of the receptor leads to an increase in intracellular cyclic AMP. CRLR mediates the vasorelaxation of arteries, suggesting a potential role for the receptor in reaction to injury, particularly in the treatment of pulmonary hypertension. CRLR expression has been reported in brain, lung, blood vessel, liver, and intestinal tract. ESTs have been isolated from B-Cell/lung/testis, bone marrow, embryo, lung, and synovium libraries.Synonyms: CALCRL, CGRPR, Calcitonin gene-related peptide type 1 receptor, Calcitonin receptor-like receptor

Gene ID: 10203

UniProt: Q16602

Pathways: cAMP Metabolic Process