HUNK antibody

Catalog No. ABIN122276

The Rabbit Polyclonal anti-HUNK antibody is suitable to detect HUNK in samples from Human. It has been validated for IHC (p).

Quick Overview for HUNK antibody (ABIN122276)

Target: HUNK (Hormonally Up-Regulated Neu-Associated Kinase (HUNK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HUNK antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HUNK Antibody

Specificity: Reacts with Hormonally Upregulated Neu Tumor-Associated Kinase (HUNK) (family: GPCR, subfamily: AMPK/SNF1)

Purification: Peptide immunogen affinity column

Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Immunohistochemistry with formalin fixed paraffin embedded tissues: 8 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.7, containing 0.01 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-80 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for HUNK

Target: HUNK (Hormonally Up-Regulated Neu-Associated Kinase (HUNK))

Alternative Name: HUNK / MAKV

Background: HUNK, an AMPK/SNF1-type protein kinase, contains a domain homologous to SNF1, a protein involved in the response to nutritional stress in yeast. HUNK has been shown to participate in vesicular transport. Additionally, functional studies in mice suggest that HUNK regulates endocytosis via interaction with rabaptin-5 protein and induces changes in mammary glands during pregnancy. HUNK expression has been documented in mouse brain, breast, embryo, and fetus. ESTs have been isolated from several human tissue libraries, including normal ear.Synonyms: B19, Hormonally up-regulated neu tumor-associated kinase, MAK-V, Serine/threonine-protein kinase MAK-V

Gene ID: 30811

UniProt: P57058