CHRM4 antibody

Catalog No. ABIN122327

The Rabbit Polyclonal anti-CHRM4 antibody is suitable to detect CHRM4 in samples from Human, Rat and Mouse. It has been validated for IHC (p).

Quick Overview for CHRM4 antibody (ABIN122327)

Target: CHRM4 (Cholinergic Receptor, Muscarinic 4 (CHRM4))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRM4 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CHRM4 Antibody

Specificity: Reacts with Muscarinic Acetylcholine Receptor M4 (family: GPCR, subfamily: Acetylcholine)

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Immunohistochemistry with formalin fixed paraffin embedded tissues: 3 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide asd preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C forlonger. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CHRM4

Target: CHRM4 (Cholinergic Receptor, Muscarinic 4 (CHRM4))

Alternative Name: Muscarinic Acetylcholine Receptor M4

Background: Muscarinic acetylcholine receptor M4 is an Acetylcholine Receptor. Unlike muscarinic receptor M2, the M4 receptor is not involved in smooth muscle contraction. The clinical relevance of this receptor is unknown. However, mouse studies have linked it to adenylyl cyclase inhibition. Muscarinic acetylcholine receptor M4 has been reported to be expressed in many regions of the rat brain, particularly the striatum and hippocampus. ESTs have been isolated from human breast and eye libraries.Synonyms: CHRM4

Gene ID: 1132

Pathways: cAMP Metabolic Process, Inositol Metabolic Process, Synaptic Membrane