CHRM4 antibody (3rd Intracellular Loop)

Catalog No. ABIN7043064

Product Details anti-CHRM4 Antibody

Target: CHRM4 (Cholinergic Receptor, Muscarinic 4 (CHRM4))

Binding Specificity: 3rd Intracellular Loop, AA 330-340

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRM4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Characteristics: Anti-CHRM4 Antibody (ABIN7043064, ABIN7044584 and ABIN7044585)) is a highly specific antibody directed against an epitope of the rat M4 muscarinic receptor. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize M4 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C) RTLNPASKWSK, corresponding to amino acid residues 330-340 of rat M4 muscarinic receptor

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.85 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CHRM4

Target: CHRM4 (Cholinergic Receptor, Muscarinic 4 (CHRM4))

Alternative Name: CHRM4 (CHRM4 Products)

Synonyms: M3R antibody, M4 antibody, HM4 antibody, M4R antibody, Chrm-4 antibody, cholinergic receptor muscarinic 4 antibody, cholinergic receptor, muscarinic 4 antibody, CHRM4 antibody, chrm4 antibody, Chrm4 antibody

Background: Alternative names: M4 Muscarinic Receptor, CHRM4, Muscarinic acetylcholine receptor M4, Cholinergic receptor muscarinic 4, mAChR M4, HM4

Gene ID: 25111

NCBI Accession: NM_000741

UniProt: P08485

Pathways: cAMP Metabolic Process, Inositol Metabolic Process, Synaptic Membrane