GRM7 antibody (Extracellular Domain, N-Term)

Catalog No. ABIN122636

The Rabbit Polyclonal anti-GRM7 antibody is suitable to detect GRM7 in samples from Human, Rat and Mouse. It has been validated for IHC (p).

Quick Overview for GRM7 antibody (Extracellular Domain, N-Term) (ABIN122636)

Target: GRM7 (Glutamate Receptor, Metabotropic 7 (GRM7))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRM7 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GRM7 Antibody

Binding Specificity: Extracellular Domain, N-Term

Specificity: This antibody detects mGluR7 / GRM7 at N-term. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GRM4 (60 %), GRM6 (45 %).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat (Others see 'Immunogen Remarks')Species reactivity (tested):Human

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Western Blot. Immunohistochemistry with formalin fixed paraffin embedded tissues: 7 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-80 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GRM7

Target: GRM7 (Glutamate Receptor, Metabotropic 7 (GRM7))

Alternative Name: mGluR7 / GRM7

Background: Metabotropic glutamate receptor GRM7 binds L-glutamate, the major excitatory neurotransmitter in the central nervous system, which activates both ionotropic and metabotropic glutamate receptors. GRM7 is highly concentrated at presynaptic terminals and is thought to play an important role in the modulation of glutamatergic synaptic transmission by effecting presynaptic inhibition of glutamate release. The metabotropic glutamate receptors are a family of G protein-coupled receptors that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group III includes GRM4, GRM6, GRM7, and GRM8 receptors, which are linked to inhibition of the cyclic AMP signaling cascade. Five mRNA variants of GRM7 have been described, most of the variants are expressed in brain and retina, although there are non-neuronal variants that may have different functional properties. GRM7 has been reported to be expressed in active zones of presynaptic axon terminals in various regions of the brain, eye, and spinal cord.Synonyms: GPRC1G, MGLUR7, Metabotropic glutamate receptor 7

Gene ID: 2917

UniProt: Q14831

Pathways: Sensory Perception of Sound, cAMP Metabolic Process, Feeding Behaviour