KRT18 antibody (Keratin 18)

Details for Product anti-KRT18 Antibody No. ABIN125989, Supplier: Log in to see
Antigen
  • CK18
  • K18
  • Krt1-18
  • CYK18
  • DreK18
  • cb83
  • dapk1
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • krt18
  • MGC64569
  • MGC75922
  • KRT18
  • keratin 18
  • keratin 18, type I L homeolog
  • keratin 18, type I
  • keratin, type I cytoskeletal 18
  • K18, simple type I keratin
  • Keratin, type I cytoskeletal 18
  • Krt18
  • KRT18
  • krt18
  • krt18.L
  • LOC700569
  • LOC100136778
  • k1c18
  • LOC100008885
Alternatives
anti-Human KRT18 antibody for Immunohistochemistry (Frozen Sections)
Reactivity
Cow (Bovine), Human
1139
248
231
136
97
90
62
58
44
43
39
34
29
29
29
27
25
24
6
4
3
2
2
1
1
1
1
Host
Mouse
883
284
14
13
4
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This KRT18 antibody is un-conjugated
61
51
29
21
20
20
18
17
14
14
14
14
14
14
14
13
13
11
11
9
8
8
8
8
8
8
8
8
8
8
8
8
8
4
3
3
3
3
3
3
3
3
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
715
564
525
462
444
305
222
170
161
120
45
41
18
14
11
10
3
3
2
1
1
1
Options
Supplier
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Immunogen Cytoskeletal preparation from tumor cell line MCF-7.
Clone Ks18-27
Isotype IgG1
Specificity Ks 18.27 represents an excellent marker to discriminate simple epithelia from those of different origin. Polypeptide reacting: Mr 45,000 polypeptide (keratin K18, formerly also designated cytokeratin 18) of all simple type and complex epithelia. Reacts with human, bovine, negative with mouse. Reacts with MCF-7, HeLa. A-431, PLC, RT 112. All adeno-CA tested, undifferentiated CA, cervix CA, hepatocellular CA.
Purification Protein A affinity chromatography
Plasmids, Primers & others Plasmids, Primers & others KRT18 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Cytokeratin 18 (KRT18 Antibody Abstract)
Background Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18
Gene ID 3875
NCBI Accession NP_000215
UniProt P05783
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes Immunohistochemistry on frozen sections: 1: 10, 1 h at RT. Cytological material. Immunoblotting. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore in 1 mL dist. water
Buffer PBS buffer, pH 7.4 containing 0.09 % sodium azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Background publications Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, 1990 (PubMed).

Moll, Achtstätter, Becht, Balcarova-Ständer, Ittensohn, Franke: "Cytokeratins in normal and malignant transitional epithelium. Maintenance of expression of urothelial differentiation features in transitional cell carcinomas and bladder carcinoma cell culture lines." in: The American journal of pathology, Vol. 132, Issue 1, pp. 123-44, 1988 (PubMed).

Moll, Franke, Schiller, Geiger, Krepler: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).