Lamin B1 antibody

Catalog No. ABIN126714

The Mouse Monoclonal anti-Lamin B1 antibody (Clone X223) (ABIN126714) specifically detects Lamin B1 in WB, IF and IHC (fro). The antibody is reactive with Human, Mouse, Rat, Cow, Xenopus laevis and Fish samples.

Quick Overview for Lamin B1 antibody (ABIN126714)

Target: Lamin B1 (LMNB1)

Reactivity: Human, Mouse, Rat, Cow, Xenopus laevis, Fish

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: X223

Product Details anti-Lamin B1 Antibody

Specificity: The monoclonal antibody decorates the karyoskeleton, i.e. the intermediate filament equivalent of the nucleus. Lamin isotypes of Mr 60-75 kDa. Xenopus laevis LII, LIII Bovine LB1, B2 Mouse LB2 Rat + Human LB1, B2 Trout LII, LIII Rat kangaroo LII, LIII

Purification: Protein A affinity chromatography.

Immunogen: Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells).

Isotype: IgG1

Application Details

Application Notes: Immunofluorescence microscopy. Immunoblotting. Immunohistochemistry: 1/10 for 1 h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1 mL distilled water.

Precaution of Use: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Lamin B1

Target: Lamin B1 (LMNB1)

Alternative Name: Lamin-B1 (LMNB1)

Background: Synonyms: LMN2, LMNB, LMNB1, LMNB2, Lamin B1/B2

Gene ID: 4001

NCBI Accession: NP_005564

UniProt: P20700

Pathways: Apoptosis, Caspase Cascade in Apoptosis