CREB1 antibody (pSer133)

Catalog No. ABIN126754

This Mouse Monoclonal antibody specifically detects CREB1 in WB and EIA. It exhibits reactivity toward Human, Mouse and Dog.

Quick Overview for CREB1 antibody (pSer133) (ABIN126754)

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Reactivity: Human, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CREB1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 1-00E-010

Product Details anti-CREB1 Antibody

Binding Specificity: pSer133

Specificity: This antibody specifically recognizes CREB phosphorylated at serine 133 and ATF1 phosphorylated at serine 63 in Western blot and ELISA applications.

Characteristics: Synonyms: cAMP Response Element-Binding protein, CREB1, CREB-1, Cyclic AMP-dependenttranscription factor ATF1

Purification: Size exclusion chromatography

Components: incl. positive Control

Immunogen: Synthetic phosphopeptide conjugated to KLH.

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CREB1

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Alternative Name: CREB1

Background: The transcription factor CREB activates transcription in response to a variety of extracellular signals. Various kinases, including Protein kinase A (PKA) , and CaM kinase II, regulate CREB activity by phosphorylation of serine 133. Molecular Weight: CREB:43 kDa, ATF1:38 kDaSynonyms: CREB-1, CREB1, Cyclic AMP-dependent transcription factor ATF1, cAMP Response Element-Binding protein

Gene ID: 1385

UniProt: P16220

Pathways: TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation