EGFR antibody (pThr678)

Catalog No. ABIN126757

This Mouse Monoclonal antibody specifically detects EGFR in WB, IP and EIA. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for EGFR antibody (pThr678) (ABIN126757)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 3F2

Product Details anti-EGFR Antibody

Binding Specificity: pThr678

Specificity: This antibody specifically recognizes EGFR phosphorylated at Threonine 678.

Characteristics: Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1

Purification: Subsequent thiophilic adsorption and size exclusion chromatography

Components: incl. positive Control

Immunogen: Phosphopeptide conjugated to KLH. Remarks: Epitope: Phosphothreonine 678, R K R pT L R R

Isotype: IgG1

Application Details

Application Notes: ELISA (0.05 μg/mL). Western blot. Immunoprecipitation (1 - 10 μg per 10^6 vanadate treated A431 cells). Luminex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Positive controlFormat: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15min with pervanadate plus PMA. Reconstitution: Reconstitute by addition of 200 µl H2O. After complete solubilization add200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90 °C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl positive control cell lysate correspond to approx. 20. 000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplicatioins with native proteins such as Immunoprecipitation. Storage: Aliquot and store frozen. Avoid repeated freeze/thaw cycles.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % Na-Azide, PEG and Sucrose, 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR / ERBB1

Background: EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

Gene ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins