EGFR antibody (pTyr1069)

Catalog No. ABIN126765

The Mouse Monoclonal anti-EGFR antibody (Clone 11C2) (ABIN126765) specifically detects EGFR in WB, IP and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for EGFR antibody (pTyr1069) (ABIN126765)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 11C2

Product Details anti-EGFR Antibody

Binding Specificity: pTyr1069

Specificity: This antibody specifically recognizes EGFR phosphorylated at Tyrosine 1069.

Characteristics: Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Components: incl. positive Control

Immunogen: Phosphopeptide conjugated to KLH. Epitope: Phosphotyrosine pY1069 (L Q R pY S S D).

Isotype: IgG1

Application Details

Application Notes: ELISA: Use at 0.05 μg/mLImmunoblotting.0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Immunoprecipitation: Use at 1-10 μg per 10^6 pervanadate-treated A431 cells. Positive Control: Cell lysate from pervanadate-treated HepG2 cells (See Protocols for moredetails).

Protocol: Positive Control Provided. Cell lysate from pervanadate-treated HepG2 cellsFormat: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15min with pervanadate. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (minigel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide/PEG and Sucrose/50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR / ERBB1

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma. The phosphorylation status of Tyrosine 1069 regulates the degradation of activated EGFR. Autophosphorylation of Tyrosine 1069 creates a docking site for the c-cbl ubiquitination ligase.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

Gene ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins