MAPK10 antibody

Catalog No. ABIN126824

The Mouse Monoclonal anti-MAPK10 antibody has been validated for WB. It is suitable to detect MAPK10 in samples from Human.

Quick Overview for MAPK10 antibody (ABIN126824)

Target: MAPK10 (Mitogen-Activated Protein Kinase 10 (MAPK10))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAPK10 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 4G6

Product Details anti-MAPK10 Antibody

Specificity: Antibody AM00080PU-N specifically interacts with the N-terminus of SAPK1 beta/jnk3 kinase. Shows no cross-reactivity with jnk1 or jnk2

Characteristics: Synonyms: Stress-activated protein kinase JNK3, c-Jun N-terminal kinase 3, JNK3A, PRKM10, MAPkinase p49 3F12, Mitogen-activated protein kinase 10

Purification: Purified from serum-free cell culture supernatant by subsequent ultrafiltration and size exclusion chromatography

Components: incl. positive Control

Immunogen: Peptide conjugated to hemocyaninEpitope: N-terminusRemarks: Molecular weight of antigen 54 kDa

Isotype: IgG

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Use cell lysate from untreated SH-SY5Y cells delivered with this product as positive control: see protocol below!
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Positive Control Cell Lysate: Untreated SH-SY5YFormat: Lyophilized cell lysate from serum starved SH-SY5Y cells. Reconstitute by addition of 200 µl H20. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Applications: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -20 °C to -80 °C. Thaw aliquots at 37 °C. Thawed aliquots may be stored at2-8 °C up to 3 months. Avoid repeated freeze / thaw cycles.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for MAPK10

Target: MAPK10 (Mitogen-Activated Protein Kinase 10 (MAPK10))

Alternative Name: MAPK10

Background: Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines. The SAPK1/jnk family consists of 3 isoforms: SAPK1alpha/jnk2, SAPK1beta/jnk3, SAPK1gamma/jnk1.Synonyms: JNK3A, MAP kinase p49 3F12, Mitogen-activated protein kinase 10, PRKM10, Stress-activated protein kinase JNK3, c-Jun N-terminal kinase 3

Gene ID: 5602

UniProt: P53779

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades