LEF1 antibody

Catalog No. ABIN126828

The Mouse Monoclonal anti-LEF1 antibody (Clone 2D12) (ABIN126828) specifically detects LEF1 in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for LEF1 antibody (ABIN126828)

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LEF1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 2D12

Product Details anti-LEF1 Antibody

Specificity: This antibody specifically recognizes LEF at 55-57 kDa. It does not crossreact with TCF3 and TCF4.

Characteristics: Synonyms: Lymphoid enhancer-binding factor 1, LEF-1, T cell-specific transcription factor 1-alpha,TCF1-alpha

Purification: Size exclusion chromatography

Immunogen: Recombinant LEF

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1mg/mL

Buffer: PBS/0.09 % Na-Azide/PEG and Sucrose/50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Alternative Name: LEF1

Background: LEF is a transcription factor of the LEF/TCF protein family. LEF interacts with cytoplasmic beta-catenin and regulates the expression of genes containing a LEF/TCF binding site. The formation of LEF - beta-catenin heterodimers is an important step in the wnt/wg signal transduction pathway.Synonyms: LEF-1, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha

Gene ID: 51176

UniProt: Q9UJU2

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding