MEK1 antibody (pSer218, pSer222, pSer226)

Catalog No. ABIN126836

The Mouse Monoclonal anti-MEK1 antibody is suitable to detect MEK1 in samples from Human, Mouse and Dog. It has been validated for WB and EIA.

Quick Overview for MEK1 antibody (pSer218, pSer222, pSer226) (ABIN126836)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivity: Human, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MEK1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 7-00E-010

Product Details anti-MEK1 Antibody

Binding Specificity: pSer218, pSer222, pSer226

Specificity: AM00090PU-N specifically recognizes MEK1 phosphorylated at serine 218/222 and MEK2 phosphorylated at serine 222/226.

Characteristics: Synonyms: MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK1, PRKMK1, MAP kinase kinase 1,Dual specificity mitogen-activated protein kinase kinase 1

Purification: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.

Components: incl. positive Control

Immunogen: Phosphopeptide conjugated to KLH. Epitope: pSer 218/222 in MEK1 pSer 222/226 in MEK2AA Sequence: D pS M A N pS FRemarks: Mol. Weight of Antigen: 45 kDa

Isotype: IgG1

Application Details

Application Notes: Suitable for Western Blot (0.5 μg/mL for HRPO/ECL detection)Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Use SW480 pervanadate-treated as positive control (see Protocols below). ELISA: 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Contains Positive Control (pervanadate treated SW 480 cells)Format: Lyophilized cell lysate from SW480 cells. Serum starved cells were treated for15min with pervanadate. Reconstitution: Add 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGEsample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRP/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation. Aliquot and store frozen. Avoid repeated freeze/thaw cycles.

Restrictions: For Research Use only

Handling

Reconstitution: Reconstitute with 1 mL H2O (15 min, RT)

Buffer: 1 x PBS / 0.09 % Na-azide / PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.

Expiry Date: 12 months

Target Details for MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MAP2K1

Background: MEK (MAP Kinase Kinase) phosphorylates the MAP Kinase 1 and 2 on both threonine and tyrosine residues of the activation loop motif TEY. MEK1 and MEK2 are activated by phosphorylation of two serine residues (Ser 218/222 in MEK1 and Ser 222/226 in MEK2). These phosphorylation sites are substrates of the Raf family of kinases.Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1

Gene ID: 5604

UniProt: Q02750

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling