AKT2 antibody

Catalog No. ABIN126856

This Mouse Monoclonal antibody specifically detects AKT2 in WB, EIA and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for AKT2 antibody (ABIN126856)

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AKT2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 8B7

Product Details anti-AKT2 Antibody

Specificity: This antibody specifically recognizes the beta isoform of PKB (PKBβ/Akt2).

Characteristics: Synonyms: RAC-PK-beta, Protein kinase Akt-2, Protein kinase B beta, RAC-betaserine/threonine-protein kinase

Purification: Thiophilic Adsorption and Size Exclusion Chromatography.

Components: incl. positive Control

Immunogen: Synthetic peptide conjugated to KLH

Isotype: IgG2a

Application Details

Application Notes: Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL, 2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for AKT2

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Alternative Name: AKT2 / PKB beta

Background: Akt, protein kinase B (PKB), is a serine/threonine kinase, which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers. AKT2 encodes a RAC/AKT-type protein kinase that contains a N-terminal pleckstrin-homology (PH) domain and a central catalytic domain closely related to both cAMP-dependent protein kinase and protein kinase C. The protein is a member of PI3K-mediated signalling pathways associated with the regulation of proliferation, survival, protein synthesis, and metabolism. It is activated by a variety of growth factors. AKT2 has been shown to be transcriptionally regulated by MyoD and to activate MyoD-myocyte enhancer binding factor-2 (MEF2) transactivation activity during muscle differentiation. Glycogen synthase kinase 3 (GSK-3) also has been shown to be a downstream target of AKT2. The AKT2 gene is one of the human homologues of v-akt, the transduced oncogene of the AKT8 virus, which induces lymphomas in mice. It has been implicated in breast, ovarian, and pancreatic cancers.Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase

Gene ID: 208

UniProt: P31751

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling