PTK2B antibody (pTyr402)
-
- Target See all PTK2B Antibodies
- PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))
-
Binding Specificity
- pTyr402
-
Reactivity
- Human, Mouse, Rat
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This PTK2B antibody is un-conjugated
-
Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificity
- This antibody specifically interacts with activated Pyk2/CAKβ phosphorylated at tyrosine 402. The antibody does not crossreact with the non-phosphorylated form of Pyk2/CAKβ nor with unrelated phosphorylation sites.
- Characteristics
- Synonyms: PYK2, RAFTK, Protein tyrosine kinase 2 beta, Focal adhesion kinase 2, CAK-beta, CADTK,Proline-rich tyrosine kinase 2, Cell adhesion kinase beta, Calcium-dependent tyrosinekinase, Related adhesion focal tyrosine kinase
- Purification
- Size exclusion chromatography
- Immunogen
- Synthetic phosphopeptide conjugated to KLH
- Clone
- 14F6
- Isotype
- IgG1
- Top Product
- Discover our top product PTK2B Primary Antibody
-
-
- Application Notes
-
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Reconstitution
- Restore with 1 mL H2O (15 min, RT).
- Buffer
- 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C/-80 °C
- Storage Comment
-
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. - Expiry Date
- 12 months
-
- Target
- PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))
- Alternative Name
- FAK2 / PTK2B (PTK2B Products)
- Synonyms
- CADTK antibody, CAKB antibody, FADK2 antibody, FAK2 antibody, PKB antibody, PTK antibody, PYK2 antibody, RAFTK antibody, CAKbeta antibody, E430023O05Rik antibody, Raftk antibody, Pyk2 antibody, fak1b antibody, ptk2.2 antibody, ptk2b antibody, si:ch1073-355e8.1 antibody, protein tyrosine kinase 2 beta antibody, PTK2 protein tyrosine kinase 2 beta antibody, protein tyrosine kinase 2aa antibody, PTK2B antibody, Ptk2b antibody, ptk2aa antibody
- Background
- Proline-rich tyrosine kinase 2 (Pyk2, also known as cellular adhesion kinase β, CAK β) is a 116 kDa cytoplasmic tyrosine kinase. Pyk2 is rapidely phosphorylated on tyrosine residues in response to activation by G-protein-coupled receptors as well as stress signals. Phosphorylation of Pyk2 leads to activation of stress-activated kinase 1 and 2 (SAPK1 and SAPK2).Synonyms: CADTK, CAK-beta, Calcium-dependent tyrosine kinase, Cell adhesion kinase beta, Focal adhesion kinase 2, PYK2, Proline-rich tyrosine kinase 2, Protein tyrosine kinase 2 beta, RAFTK, Related adhesion focal tyrosine kinase
- Gene ID
- 2185
- UniProt
- Q14289
- Pathways
- EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGF Signaling
-