Vimentin antibody
Quick Overview for Vimentin antibody (ABIN126914)
Target
See all Vimentin (VIM) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Specificity
- This antibody specifically recognizes Vimentin at 54 kDa in Western-blot applications.
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Purification
- Size exclusion chromatography
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Components
- incl. positive Control
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Immunogen
- Synthetic peptide conjugated to hemocyanin
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Isotype
- IgG
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anti-Vimentin (VIM) (AA 371-466) antibody
VIM Reactivity: Human, Rat, Mouse, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IF (p), IHC (fro), IF (cc) Host: Rabbit Polyclonal unconjugated
anti-Vimentin (VIM) (AA 435-466), (C-Term) antibodyVIM Reactivity: Human, Rat, Mouse WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Vimentin (VIM) antibodyKO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Vimentin (VIM) (AA 1-200) antibodyVIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Vimentin (VIM) antibodyVIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated
anti-Vimentin (VIM) antibodyVIM Reactivity: Human, Rat, Mouse, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated
anti-Vimentin (VIM) antibodyVIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated
anti-Vimentin (VIM) antibodyVIM Reactivity: Human WB, IHC, IF Host: Mouse Monoclonal C12 unconjugated
anti-Vimentin (VIM) (AA 41-140) antibodyVIM Reactivity: Human WB, ELISA, IF, IP Host: Mouse Monoclonal 3E9 unconjugated
anti-Vimentin (VIM) (AA 210-466) antibodyVIM Reactivity: Human WB, IHC, IHC (p) Host: Mouse Monoclonal 5D7 unconjugated
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Application Notes
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Reconstitution
- Restore with 1 mL H2O (15 min, RT).
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Buffer
- 1 mL2 x PBS containing 0.09 % Sodium Azide/PEG and Sucrose.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C/-80 °C
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Storage Comment
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. -
Expiry Date
- 12 months
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- Vimentin (VIM)
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Alternative Name
- Vimentin
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Background
- Vimentin is a major subunit protein of intermediate filaments found in mesenchymal cells. Vimentin is expressed in many tumors/tumor cell lines after epithelial to mesenchymal transition.Synonyms: VIM
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Molecular Weight
- 54 kDa
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Gene ID
- 7431
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UniProt
- P08670
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Pathways
- Caspase Cascade in Apoptosis
Target
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