FADD antibody

Catalog No. ABIN126919

This Mouse Monoclonal antibody specifically detects FADD in WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for FADD antibody (ABIN126919)

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This FADD antibody is un-conjugated

Application: Western Blotting (WB)

Clone: T1B

Product Details anti-FADD Antibody

Specificity: The antibody detects 28 kDa protein, corresponding to the apparent molecular weight of FADD in SDS-PAGE immunoblots.

Characteristics: Synonyms: FAS-associated death domain protein, MORT1, GIG3, FAS-associating deathdomain-containing protein, Mediator of receptor induced toxicity, Growth-inhibiting gene 3protein

Purification: Affinity chromatography

Immunogen: Recombinant human FADD

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1-4 μg/mL, HeLa cell lysate can be used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS containing 30 % glycerol, 0.5 % BSA and 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C to -70 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for FADD

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Alternative Name: FADD

Background: Fas-associating death domain containing protein (FADD), also known as mediator of receptor induced toxicity (MORT1), is a 24 kDa cytosolic protein. The protein is involved in both Fas and TNF-R induced cell death pathway. FADD specifically binds to Fas, an association mediated by their homologous death domains. Overexpression of FADD induces apoptosis that can be inhibited by CrmA, a poxvirus protein that blocks both Fas- and TNF-induced cell death.Synonyms: FAS-associated death domain protein, FAS-associating death domain-containing protein, GIG3, Growth-inhibiting gene 3 protein, MORT1, Mediator of receptor induced toxicity

Gene ID: 8772

UniProt: Q13158

Pathways: Apoptosis, TLR Signaling, Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades