Perforin 1 antibody

Catalog No. ABIN126932

The Mouse Monoclonal anti-Perforin 1 antibody is suitable to detect Perforin 1 in samples from Human. It has been validated for IP, FACS, IF and IHC (fro).

Quick Overview for Perforin 1 antibody (ABIN126932)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Perforin 1 antibody is un-conjugated

Application: Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: DG9

Product Details anti-Perforin 1 Antibody

Specificity: Recognizes the 70 kDa Perforin.

Characteristics: Synonyms: Perforin-1, Pore forming protein, PFN, PFP, Cytolysin, Lymphocyte pore-forming protein

Purification: Affinity Chromatography.

Immunogen: Purified granules from Human YT lymphoma cells.

Isotype: IgG2b

Application Details

Application Notes: Immunoprecipitation: 2-8 μg/mL. Flow Cytometry: 2-8 μg/mL. Immunohistochemistry: 2-8 μg/mL. Immunocytochemistry (Frozen and Paraffin): 2-8 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 1 mg/mL BSA and 1.5 mM Thimerosal and 50 % Glycerol.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: Perforin 1 / PRF1

Background: Human Perforin (Cytolysin) is believed to be one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis. Perforin expression is constitutive on NK cells, but increases in resting XD8+ cytotoxic cells upon activation.Synonyms: Cytolysin, Lymphocyte pore-forming protein, PFN, PFP, Perforin-1, Pore forming protein

Gene ID: 5551

UniProt: P14222

Pathways: Apoptosis, Caspase Cascade in Apoptosis