Caspase 1 antibody

Catalog No. ABIN126963

The Rabbit Polyclonal anti-Caspase 1 antibody (ABIN126963) specifically detects Caspase 1 in WB, IP and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Caspase 1 antibody (ABIN126963)

Target: Caspase 1 (CASP1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-Caspase 1 Antibody

Specificity: This antibody recognizes 45 kDa Procaspase-1 and the 20 kDa cleaved product.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide surrounding amino acid 132 of Human Caspase-1.

Application Details

Application Notes: Western Blot (0.5-4 μg/mL). Immunoprecipitation (4-8 μg/mL). Immunofluorescence (10-20 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 30 % Glycerol, 0.5 % BSA as stabilizer and 0.01 % Thimerosal as preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for Caspase 1

Target: Caspase 1 (CASP1)

Alternative Name: Caspase-1

Background: Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase 1 is similar to the cell death gene CED3 of C. elegans and regulates multiple proinflammatory cytokines, including Interleukin 1b and interferon-gamma-inducing factor. Caspase 1 plays a role down stream of Caspase 8 which is involved in Fas-mediated apoptosis.Synonyms: CASP-1, CASP1, ICE, IL1BC, IL1BCE, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, p45

Gene ID: 834

NCBI Accession: NP_001214

UniProt: P29466

Pathways: Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome