BAX antibody (N-Term)

Catalog No. ABIN126979

This Rabbit Polyclonal antibody specifically detects BAX in WB and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BAX antibody (N-Term) (ABIN126979)

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAX antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-BAX Antibody

Binding Specificity: N-Term

Specificity: The antibody detects a 20 kDa protein, corresponding to the apparent molecular weight of Bax on SDS-PAGE Immunoblots.

Purification: Affinity Chromatography

Immunogen: Synthetic peptide corresponding to amino acids near the N-terminus of Human Bax

Application Details

Application Notes: Western BLot: 0.5-4 μg/mL. Immunoprecipitation: 10-20 μg/mL. Rat kidney tissue lysate can be used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: Bax

Background: The Bcl-2 (B-cell leukemia/lymphoma 2) family proteins function to either inhibit (Bcl-2 and Bcl-XL) or promote (Bax and Bak) apoptosis. Bax contains the conserved Bcl-2 homology 1 (BH1) and 2 (BH2) domains that allow for its homodimerization or heterodimerization with Bcl-2. When Bcl-2 is in excess, apoptosis is inhibited. However, if Bax levels increase in response to a death signal, the cell is pushed toward death.Synonyms: Apoptosis regulator BAX, BCL2-associated X protein, BCL2L4, Bcl-2-like protein 4, Bcl2-L-4

Gene ID: 9606

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response