FADD antibody

Catalog No. ABIN126986

The Rabbit Polyclonal anti-FADD antibody is suitable to detect FADD in samples from Human, Mouse and Rat. It has been validated for WB and IP.

Quick Overview for FADD antibody (ABIN126986)

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FADD antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-FADD Antibody

Specificity: The antibody detects an ~28 kDa band, corresponding to the expected molecular mass of FADD on immunoblots. The antibody specificity is confirmed by specific peptide inhibition in immunoblotting studies.

Purification: Affinity chromatography

Immunogen: Synthetic peptide surrounding amino acid 128 of human FADD

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL. Based on researcher's feedback, it can also be used in immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C to -70 °C.

Target Details for FADD

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Alternative Name: FADD

Background: Fas-associating death domain containing protein (FADD), also known as mediator of receptor induced toxicity (MORT1), is a 23 kDa cytosolic protein with an apparent molecular weight of 28 kDa on SDS-PAGE. The protein is involved in both Fas and TNF-R induced cell death pathway. FADD specifically binds to Fas to initiate the apoptotic signalling cascade. Overexpression of FADD induces apoptosis.Synonyms: FAS-associated death domain protein, FAS-associating death domain-containing protein, GIG3, Growth-inhibiting gene 3 protein, MORT1, Mediator of receptor induced toxicity

Gene ID: 8772

NCBI Accession: NP_003815

UniProt: Q13158

Pathways: Apoptosis, TLR Signaling, Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades