WEE1 antibody (C-Term)

Catalog No. ABIN127016

The Rabbit Polyclonal anti-WEE1 antibody has been validated for WB and IP. It is suitable to detect WEE1 in samples from Human, Mouse and Rat.

Quick Overview for WEE1 antibody (C-Term) (ABIN127016)

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WEE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-WEE1 Antibody

Binding Specificity: C-Term

Specificity: The antibody recognizes Wee1.

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide mapping to the C-terminus of human Wee1.

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL. Mouse small intestine tissue lysate can be used as a positive control. Immunoprecipitation: 5-10 μg/mL. Not suitable for Paraffin Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or (in aliquots) at -70 °C for longer.

Target Details for WEE1

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Alternative Name: WEE1 / Wee1-Like Protein Kinase

Background: Wee1 is a nuclear tyrosine protein kinase. The protein plays a role in suppressing eukaryotes' entry into mitosis by inactivating cdc2 kinase by inhibiting tyrosine phosphorylation. The predicted molecular weight of the kinase is 72 kDa. The kinase possesses tyrosine kinase activity, but structurally belongs to the serine/threonine protein kinase family.Synonyms: WEE1hu, Wee1A kinase

Gene ID: 7465

NCBI Accession: NP_003381

UniProt: P30291

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase