Caspase 7 antibody (Cleavage Site)

Catalog No. ABIN127023

The Rabbit Polyclonal anti-Caspase 7 antibody (ABIN127023) specifically detects Caspase 7 in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Caspase 7 antibody (Cleavage Site) (ABIN127023)

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caspase 7 Antibody

Binding Specificity: Cleavage Site

Specificity: The antibody detects the full length (35-37 kDa) and the large fragment (20 kDa) of processed caspase-7 by Western blotting.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to residues surrounding the cleavage site of human caspase-7 large fragment

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for Caspase 7

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Alternative Name: Caspase-7

Background: Caspase-7 is a 34 kDa protein consisting of two subunits and a short prodomain. The procaspase-7 can be activated by cleavage by upstream caspases to form active caspase-7 in apoptosis. Once activated, caspase-7 cleaves many of the same substrates as caspase-3, including poly (ADP-ribose) polymerase (PARP) and sterol regulatory element binding proteins (SREBPs).Synonyms: Apoptotic protease Mch-3, CASP-7, CASP7, CMH-1, ICE-like apoptotic protease 3, MCH3

Gene ID: 840

NCBI Accession: NP_001218

UniProt: P55210

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity