PAWR antibody

Catalog No. ABIN127126

This Rabbit Polyclonal antibody specifically detects PAWR in WB, IHC (p) and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PAWR antibody (ABIN127126)

Target: PAWR (PRKC, Apoptosis, WT1, Regulator (PAWR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAWR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-PAWR Antibody

Specificity: The antibody recognizes PAR4.

Purification: Peptide affinity purified

Immunogen: Synthetic peptide surrounding amino acid 327 of human Par4.

Application Details

Application Notes: Western Blot: 1-4 μg/mL. Immunoprecipitation: 20 μg/mL. Immunohistochemistry: 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS containing 50 % glycerol, 1 % BSA, and 0.02 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for PAWR

Target: PAWR (PRKC, Apoptosis, WT1, Regulator (PAWR))

Alternative Name: PAR4 / PAWR

Background: Five ionomycin-inducible complementary cDNAs, designated PAR1, 2, 3, 4 and 5, have been isolated from the prostate cancer cell line AT-3. Nucleotide sequencing identified PAR1 as the rat homolog of MKP-1, PAR2 as the injury-inducible gene HB-EGF, and PAR3 as the serum-induced gene cyr-61. PAR4 and PAR5 sequences were not found to correspond to any previously described proteins. PAR4 (prostate apoptosis response-4) is specifically expressed by cells entering apoptosis and is not inducible by growth factor stimulation, oxidative stress and necrosis, or growth arrest. The PAR4 gene encodes a 38 kDa protein with a putative nuclear localization signal and carboxy terminal leucine zipper.Synonyms: PRKC apoptosis WT1 regulator protein, Par-4, Prostate apoptosis response 4 protein

Gene ID: 5074

NCBI Accession: NP_002574

UniProt: Q96IZ0