LIMK2 antibody (C-Term)

Catalog No. ABIN127209

The Rabbit Polyclonal anti-LIMK2 antibody (ABIN127209) specifically detects LIMK2 in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for LIMK2 antibody (C-Term) (ABIN127209)

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIMK2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LIMK2 Antibody

Binding Specificity: C-Term

Specificity: The antibody detects 70 kDa LIMK2 protein. It does not cross-react with LIMK1. The antibody recognizes ~35 kDa PHAP III.

Purification: Affinity Purified

Immunogen: Synthetic peptide corresponding to the C-terminus of human LIMK2.

Application Details

Application Notes: Western Blot: 1-4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for LIMK2

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Alternative Name: LIMK2

Background: LIM kinases (LIMK1 and LIMK2) are serine/threnine kinases that have two zinc finger motifs, known as LIM motifs in their N-terminal regulatory domain. LIM kinases are involved in actin cytoskeleton regulation through Rho-family GTPases and downstream kinases PAKs and ROCK. PAK1 and ROCK phosphorylate LIMK1 and LIMK2, which increases the activity of the kinases. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the N-terminus of Cofilin.Synonyms: EC=2.7.11.1, LIM domain kinase 2, LIMK-2, LIMK2

Gene ID: 3985

NCBI Accession: NP_005560

UniProt: P53671