Phospholipase C gamma 2 antibody (C-Term)

Catalog No. ABIN127211

The Rabbit Polyclonal anti-Phospholipase C gamma 2 antibody is suitable to detect Phospholipase C gamma 2 in samples from Human, Rat and Mouse. It has been validated for WB, IHC (p) and IP.

Quick Overview for Phospholipase C gamma 2 antibody (C-Term) (ABIN127211)

Target: Phospholipase C gamma 2 (PLCG2)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase C gamma 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-Phospholipase C gamma 2 Antibody

Binding Specificity: C-Term

Specificity: The antibody recognizes 150 kDa PLCγ2. The antibody does not cross-react with PLCγ1.

Purification: Affinity purified

Immunogen: Synthetic peptide near the C-terminus of human PLCgamma2.

Application Details

Application Notes: Western Blot: 1-4 μg/mL. Immunoprecipitation: 20 μg/mL. Immunohistochemistry: 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for Phospholipase C gamma 2

Target: Phospholipase C gamma 2 (PLCG2)

Alternative Name: PLCG2

Background: PLC (Phosphoinositide-specific phospholipase C) plays a significant role in transmembrane signaling. Four members of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. In response to extracellular stimuli (e.g., hormone, growth factors, neurotransmitters), PLC hydrolizes phosphatidylinositol 4,5-biphosphate (PIP2) into two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). PLCγ2 is engaged in antigen-dependent signaling in B-cells and collagen-dependent signaling in platelets.Synonyms: 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-2, PLC-IV, PLC-gamma-2, Phosphoinositide phospholipase C-gamma-2, Phospholipase C-IV, Phospholipase C-gamma-2

Gene ID: 5336

NCBI Accession: NP_002652

UniProt: P16885

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades, VEGF Signaling, BCR Signaling