Reactivity: Human
WB, ChIP, DB, ChIP-seq
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western Blot: 1 μg/mL. Immunoprecipitation: 20-30 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C/-80 °C
Storage Comment
Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.
Target
Histone H2B
Background
The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.Synonyms: H2B Histone Family, HIST1H2B