HDAC4 antibody

Catalog No. ABIN127262

The Rabbit Polyclonal anti-HDAC4 antibody is suitable to detect HDAC4 in samples from Human and Chicken. It has been validated for WB.

Quick Overview for HDAC4 antibody (ABIN127262)

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Reactivity: Human, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HDAC4 Antibody

Specificity: The antibody recognizes L-Selectin.

Purification: Affinity purified

Immunogen: Synthetic peptide surrounding amino acid 10 of human HDAC-4.

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for HDAC4

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Alternative Name: HDAC4

Background: HDAC4 is a member of the class II mammalian histone deacetylases, which consists of 1084 amino acid residues. Its C-terminal sequence is highly similar to the deacetylase domain of yeast HDA1. HDAC4, unlike other deacetylases, shuttles between the nucleus and cytoplasm in a process involving active nuclear export. Association of HDAC4 with 14-3-3 results in sequestration of HDAC 4 protein in the cytoplasm. In the nucleus, HDAC4 associates with the myocyte enhancer factor MEF2A. Binding of HDAC4 to MEF2A results in the repression of MEF2A transcriptional activation. HDAC4 has also been shown to interact with other deacetylases such as HDAC3 as well as the corepressors NcoR and SMART.Synonyms: HD4, Histone deacetylase 4, KIAA0288

Gene ID: 9759

NCBI Accession: NP_006028

UniProt: P56524

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process