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Vimentin antibody

This Rabbit Polyclonal antibody specifically detects Vimentin in WB, IHC (p) and IP. It exhibits reactivity toward Human, Rat, Mouse, Cow and Chicken.
Catalog No. ABIN127264
$594.00
Plus shipping costs $50.00
0.1 mg
Shipping to: United States
Delivery in 1 to 2 Business Days

Quick Overview for Vimentin antibody (ABIN127264)

Target

See all Vimentin (VIM) Antibodies
Vimentin (VIM)

Reactivity

  • 392
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  • 74
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  • 50
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Human, Rat, Mouse, Cow, Chicken

Host

  • 204
  • 169
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  • 2
Rabbit

Clonality

  • 225
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Polyclonal

Conjugate

  • 240
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This Vimentin antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
  • Specificity

    Recognizes Vimentin (50-55 kDa).

    Purification

    Affinity Purified

    Immunogen

    Synthetic peptide surrounding amino acid 448 of Human Vimentin.
  • Application Notes

    Western Blot: 0.5-4 μg/mL. Immunoprecipitation: 10-20 μg/mL. Immunohistochemistry: 10-20 μg/mL. Positive Control: Mouse small intestine tissue lysate.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    0.2 mg/mL

    Buffer

    Phosphate-buffered saline (PBS) containing 30 % Glycerol, 0.5 % BSA as stabilizer and 0.01 % Thimerosal as preservative

    Preservative

    Thimerosal (Merthiolate)

    Precaution of Use

    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    -20 °C/-80 °C

    Storage Comment

    Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -20 °C to -70 °C.
  • Target

    Vimentin (VIM)

    Alternative Name

    Vimentin

    Background

    Vimentin, a intermediate filament protein, is a general marker of cells originating in the mesenchyme. Vimentin is frequently co-expressed with other members of the intermediate filament family such as the cytokeratins, in neoplasm.Synonyms: VIM

    Gene ID

    7431

    NCBI Accession

    NP_003371

    UniProt

    P08670

    Pathways

    Caspase Cascade in Apoptosis
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