Vimentin antibody
Quick Overview for Vimentin antibody (ABIN127264)
Target
See all Vimentin (VIM) AntibodiesReactivity
Host
Clonality
Conjugate
Application
-
-
Specificity
- Recognizes Vimentin (50-55 kDa).
-
Purification
- Affinity Purified
-
Immunogen
- Synthetic peptide surrounding amino acid 448 of Human Vimentin.
-
-
-
-
Application Notes
-
Western Blot: 0.5-4 μg/mL. Immunoprecipitation: 10-20 μg/mL. Immunohistochemistry: 10-20 μg/mL. Positive Control: Mouse small intestine tissue lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
-
-
-
Concentration
- 0.2 mg/mL
-
Buffer
- Phosphate-buffered saline (PBS) containing 30 % Glycerol, 0.5 % BSA as stabilizer and 0.01 % Thimerosal as preservative
-
Preservative
- Thimerosal (Merthiolate)
-
Precaution of Use
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Handling Advice
- Avoid repeated freezing and thawing.
-
Storage
- -20 °C/-80 °C
-
Storage Comment
- Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -20 °C to -70 °C.
-
-
- Vimentin (VIM)
-
Alternative Name
- Vimentin
-
Background
- Vimentin, a intermediate filament protein, is a general marker of cells originating in the mesenchyme. Vimentin is frequently co-expressed with other members of the intermediate filament family such as the cytokeratins, in neoplasm.Synonyms: VIM
-
Gene ID
- 7431
-
NCBI Accession
- NP_003371
-
UniProt
- P08670
-
Pathways
- Caspase Cascade in Apoptosis
Target
-