C-JUN antibody

Catalog No. ABIN127313

The Rabbit Polyclonal anti-C-JUN antibody is suitable to detect C-JUN in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for C-JUN antibody (ABIN127313)

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C-JUN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-C-JUN Antibody

Specificity: The antibody only recognizes c-Jun, and will not detect the endogenous levels of Jun B or Jun D.

Purification: Affinity Purified

Application Details

Application Notes: Western blotting (0.5-2 μg/mL). Immunohistochemistry (20-40 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 50 % glycerol, 1 % BSA and 0.02 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20 °C. For long-term storage, aliquot and freeze at -70 °C.

Target Details for C-JUN

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Alternative Name: AP-1 / C-Jun

Background: Extracellular stimuli such as growth factors and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation at Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. JNK (c-jun N-terminal Kinase) binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. The activity of JNK is stimulated by the same signals that activate c-Jun.Synonyms: Activator protein 1, Proto-oncogene c-jun, Transcription factor AP1, V-jun avian sarcoma virus 17 oncogene homolog, p39

Gene ID: 3725

NCBI Accession: NP_002219

UniProt: P05412

Pathways: MAPK Signaling, RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins