POLM antibody (AA 6-18)

Catalog No. ABIN129588

Supplier Product No.: 600-401-462

This anti-POLM antibody is a Rabbit Polyclonal antibody detecting POLM in WB and ELISA. Suitable for Human.

Quick Overview for POLM antibody (AA 6-18) (ABIN129588)

Target: POLM (Polymerase (DNA Directed), mu (POLM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLM antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-POLM Antibody

Binding Specificity: AA 6-18

Supplier Product No.: 600-401-462

Supplier: Rockland

Purpose: POLM Mu Antibody

Characteristics: Synonyms: rabbit anti-POLM antibody, DNA-directed DNA/RNA polymerase mu , Pol Mu, Terminal transferase, DNA Polymerase Mu antibody, DNA-directed DNA polymerase mu antibody, Pol iota antibody

Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 6-18 of Human POLM m (DNA polymerase mu).  POLM mu is a template-directed terminal deoxynucleotide transferase that shows a preferential expression in proliferating lymphoid cells.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA against the immunizing peptide.  Reactivity in other immunoassays is unknown.

Western Blot Dilution: 1:500- 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for POLM

Target: POLM (Polymerase (DNA Directed), mu (POLM))

Alternative Name: POLM

Background: Background: DNA-directed DNA/RNA polymerase mu (POLM) is a Gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). It participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination. DPOLM has a reduced ability to distinguish dNTP and rNTP as substrates, and elongates them on DNA primer strand with a similar efficiency. It is able to polymerize nucleotides on RNA primer strands.

Gene ID: 27434

UniProt: Q9NP87

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response