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GLI3 antibody (AA 41-57)

GLI3 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN129623
  • Target See all GLI3 Antibodies
    GLI3 (GLI Family Zinc Finger 3 (GLI3))
    Binding Specificity
    • 7
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 41-57
    Reactivity
    • 45
    • 23
    • 16
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 36
    • 7
    • 3
    Rabbit
    Clonality
    • 32
    • 14
    Polyclonal
    Conjugate
    • 25
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    This GLI3 antibody is un-conjugated
    Application
    • 17
    • 13
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Cross-Reactivity
    Chimpanzee, Squirrel Monkey (Saimiri spec.), Xenopus laevis, Chicken, Dog (Canine), Quail
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Immunogen
    This affinity purified antibody was produced from monospecific rabbit serum by repeated immunizations with a synthetic peptide corresponding to amino acids 41-57 of human Gli-3 protein.
    Isotype
    IgG
    Top Product
    Discover our top product GLI3 Primary Antibody
  • Application Notes
    This antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Detection of Gli-3 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-3 is likely to be low. Furthermore, Gli3 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
  • Hehemann, Kalmanek, Choe, Dynda, Hu, Quek, Harrington, Stupp, McVary, Podlasek: "Sonic hedgehog regulation of human rhabdosphincter muscle:Potential implications for treatment of stress urinary incontinence." in: Neurourology and urodynamics, Vol. 37, Issue 8, pp. 2551-2559, (2019) (PubMed).

  • Target
    GLI3 (GLI Family Zinc Finger 3 (GLI3))
    Alternative Name
    Gli-3 (GLI3 Products)
    Synonyms
    ACLS antibody, GCPS antibody, GLI3-190 antibody, GLI3FL antibody, PAP-A antibody, PAPA antibody, PAPA1 antibody, PAPB antibody, PHS antibody, PPDIV antibody, xgli3 antibody, AI854843 antibody, AU023367 antibody, Bph antibody, Pdn antibody, Xt antibody, add antibody, im:7142603 antibody, GLI3 antibody, GLI family zinc finger 3 antibody, GLI family zinc finger 3 L homeolog antibody, GLI-Kruppel family member GLI3 antibody, GLI3 antibody, Gli3 antibody, gli3.L antibody, gli3 antibody
    Background
    Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS); an autosomal dominant disorder-affecting limb and cranio-facial development.  Two isoforms of human Gli-3 have been reported.  One is the full-length protein at ~170-190kDa and the other is a truncated isoform at ~80kDa.
    Synonyms: Gli-3, Zinc Finger Protein Gli-3, GLI-Kruppel family member GLI-3
    Gene ID
    2737, 119393899
    UniProt
    P10071
    Pathways
    Hedgehog Signaling
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