GGA3 antibody (AA 400-425)

Catalog No. ABIN129660

Supplier Product No.: 600-401-893

Product Details anti-GGA3 Antibody

Target: GGA3 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 3 (GGA3))

Binding Specificity: AA 400-425

Reactivity: Human, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GGA3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 600-401-893

Supplier: Rockland

Purpose: GGA3 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human GGA3 protein.

Characteristics: Synonyms: rabbit anti-GGA3 antibody, GGA-3, GGA 3, ADP-ribosylation factor-binding protein GGA3, Golgi-localized gamma ear-containing ARF-binding protein 3

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 400-425 of Human GGA3.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect bands at 78 kDa in size corresponding to GGA3 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.48 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GGA3

Target: GGA3 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 3 (GGA3))

Alternative Name: GGA3 (GGA3 Products)

Background: Background: GGA3 (also known as ADP-ribosylation factor binding protein GGA3, Golgi-localized, gamma ear-containing, ARF-binding protein 3, Gamma-adaptin related protein 3) plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. This protein mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. GGA1 is a monomeric protein that interacts with SORT1, SORL1, LRP3, GGA binding partner (GGABP) and P200, GGA1 and GGA2 as well as RABEP1, RABGEF1 and type-I membrane proteins M6PR/CD-MPR, IGF2R/CI-MPR and BACE1. GGA3 is a ubiquitously expressed membrane-associated protein that is localized to TGN and endosomes. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene ID: 23163, 20336267

UniProt: Q9NZ52